Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 7, 2015
Previous Article Next Article

Characterization of zinc uptake by mouse primary cultured astrocytes and microglia

Author affiliations

Abstract

To elucidate the regulatory mechanism for extracellular zinc in the CNS, we examined the zinc uptake characteristics in mouse astrocytes and microglia. Zinc was taken up into the two cell types time-dependently, and the cell-to-medium concentration (C/M) ratio in the initial uptake phase in astrocytes was significantly smaller than that in microglia, while in the steady state phase, there was no difference in their C/M ratios. In both astrocytes and microglia, the zinc uptake was mediated, at least in part, by high- and low-affinity systems. There were no differences for both in the Km values for zinc uptake between astrocytes and microglia, and those for the low-affinity system in both cell types were the same as that for mouse ZIP1 reported previously. On the other hand, the Vmax values for both systems were greater in microglia than in astrocytes. Among ZIP isoforms, expression of ZIP1 was high in astrocytes and microglia. Nickel, a competitive inhibitor of ZIP1, and ZIP1 knock-down decreased zinc uptake by both types of cells. Overall, it is demonstrated that astrocytes and microglia had a similar uptake system for zinc including ZIP1, and the differences found in their uptake profiles imply that they play different roles in the regulation of extracellular zinc to maintain brain homeostasis.

Graphical abstract: Characterization of zinc uptake by mouse primary cultured astrocytes and microglia

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Mar 2015, accepted on 20 Apr 2015 and first published on 20 Apr 2015


Article type: Paper
DOI: 10.1039/C5MT00085H
Citation: Metallomics, 2015,7, 1067-1077

  •   Request permissions

    Characterization of zinc uptake by mouse primary cultured astrocytes and microglia

    S. Segawa, N. Tatsumi, A. Ohishi, K. Nishida and K. Nagasawa, Metallomics, 2015, 7, 1067
    DOI: 10.1039/C5MT00085H

Search articles by author

Spotlight

Advertisements