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Issue 3, 2015
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Effects of non-toxic zinc exposure on human epidermal keratinocytes

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Abstract

Zinc is an essential microelement; its importance to the skin is highlighted by the severe skin symptoms in hereditary or acquired zinc deficiency, by the improvement of several skin conditions using systemic or topical zinc preparations and by the induced intracellular zinc release upon UVB exposure, which is the main harmful environmental factor to the skin. Understanding the molecular background of the role of zinc in skin may help gain insight into the pathology of skin disorders and provide evidence for the therapeutic usefulness of zinc supplementation. Herein, we studied the effects of zinc chloride (ZnCl2) exposure on the function of HaCaT keratinocytes, and the results showed that a non-toxic elevation in the concentration of extracellular zinc (100 μM) facilitated cell proliferation and induced significant alterations in the mRNA expression of NOTCH1, IL8, and cyclooxygenase-2. In addition, increased heme oxygenase-1 (HMOX1) expression and non-toxic generation of superoxide were detected in the first 4 h. Regarding the effects on the UVB-induced toxicity, although the level of cyclobutane pyrimidine dimers in the keratinocytes pre-treated with zinc for 24 h was reduced 3 h after UVB irradiation, significantly enhanced superoxide generation was observed 10 h after UVB exposure in the zinc pre-exposed cells. The overall survival was unaffected; however, there was a decrease in the percentage of early apoptotic cells and an increase in the percentage of late apoptotic plus necrotic cells. These results suggest that the exposure of human keratinocytes to non-toxic concentrations of ZnCl2 impacts gene expression, cell proliferation and the responses to environmental stress in the skin. It would be important to further examine the role of zinc in skin and further clarify whether this issue can affect our thinking regarding the pathogenesis of skin diseases.

Graphical abstract: Effects of non-toxic zinc exposure on human epidermal keratinocytes

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Publication details

The article was received on 01 Nov 2014, accepted on 20 Jan 2015 and first published on 09 Feb 2015


Article type: Paper
DOI: 10.1039/C4MT00287C
Citation: Metallomics, 2015,7, 499-507

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    Effects of non-toxic zinc exposure on human epidermal keratinocytes

    E. Emri, E. Miko, P. Bai, G. Boros, G. Nagy, D. Rózsa, T. Juhász, C. Hegedűs, I. Horkay, É. Remenyik and G. Emri, Metallomics, 2015, 7, 499
    DOI: 10.1039/C4MT00287C

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