Jump to main content
Jump to site search

Issue 17, 2015
Previous Article Next Article

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

Author affiliations

Abstract

There is a recognized and growing need for rapid and efficient cell assays, where the size of microfluidic devices lend themselves to the manipulation of cellular populations down to the single cell level. An exceptional way to analyze cells independently is to encapsulate them within aqueous droplets surrounded by an immiscible fluid, so that reagents and reaction products are contained within a controlled microenvironment. Most cell encapsulation work has focused on the development and use of passive methods, where droplets are produced continuously at high rates by pumping fluids from external pressure-driven reservoirs through defined microfluidic geometries. With limited exceptions, the number of cells encapsulated per droplet in these systems is dictated by Poisson statistics, reducing the proportion of droplets that contain the desired number of cells and thus the effective rate at which single cells can be encapsulated. Nevertheless, a number of recently developed actively-controlled droplet production methods present an alternative route to the production of droplets at similar rates and with the potential to improve the efficiency of single-cell encapsulation. In this critical review, we examine both passive and active methods for droplet production and explore how these can be used to deterministically and non-deterministically encapsulate cells.

Graphical abstract: The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

Back to tab navigation

Article information


Submitted
03 Jun 2015
Accepted
21 Jul 2015
First published
30 Jul 2015

Lab Chip, 2015,15, 3439-3459
Article type
Critical Review
Author version available

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

D. J. Collins, A. Neild, A. deMello, A. Liu and Y. Ai, Lab Chip, 2015, 15, 3439
DOI: 10.1039/C5LC00614G

Social activity

Search articles by author

Spotlight

Advertisements