Jump to main content
Jump to site search

Issue 12, 2015
Previous Article Next Article

Non-contact acoustic capture of microparticles from small plasma volumes

Author affiliations

Abstract

Microparticles (MP) are small (100–1000 nm) membrane vesicles shed by cells as a response to activation, stress or apoptosis. Platelet-derived MP (PMP) has been shown to reflect the pathophysiological processes of a range of cardiovascular diseases and there is a potential clinical value in using PMPs as biomarkers, as well as a need to better understand the biology of these vesicles. The current method for isolating MP depends on differential centrifugation steps, which require relatively large sample volumes and have been shown to compromise the integrity and composition of the MP population. We present a novel method for rapid, non-contact capture of PMP in minute sample volumes based on a microscale acoustic standing wave technology. Capture of PMPs from plasma is shown by scanning electron microscopy and flow cytometry. Furthermore, the system is characterized with regards to plasma sample concentration and flow rate. Finally, the technique is compared to a standard differential centrifugation protocol using samples from both healthy controls and ST-elevation myocardial infarction (STEMI) patient samples. The acoustic system is shown to offer a quick and automated setup for extracting microparticles from small sample volumes with higher recovery than a standard differential centrifugation protocol.

Graphical abstract: Non-contact acoustic capture of microparticles from small plasma volumes

Back to tab navigation

Publication details

The article was received on 11 Mar 2015, accepted on 21 Apr 2015 and first published on 21 Apr 2015


Article type: Paper
DOI: 10.1039/C5LC00290G
Author version
available:
Download author version (PDF)
Lab Chip, 2015,15, 2588-2596
  • Open access: Creative Commons BY license
  •   Request permissions

    Non-contact acoustic capture of microparticles from small plasma volumes

    M. Evander, O. Gidlöf, B. Olde, D. Erlinge and T. Laurell, Lab Chip, 2015, 15, 2588
    DOI: 10.1039/C5LC00290G

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements