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Issue 11, 2015
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Differentiation of neuroepithelial stem cells into functional dopaminergic neurons in 3D microfluidic cell culture

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Abstract

A hallmark of Parkinson's disease is the progressive loss of nigrostriatal dopaminergic neurons. We derived human neuroepithelial cells from induced pluripotent stem cells and successfully differentiated them into dopaminergic neurons within phase-guided, three-dimensional microfluidic cell culture bioreactors. After 30 days of differentiation within the microfluidic bioreactors, in situ morphological, immunocytochemical and calcium imaging confirmed the presence of dopaminergic neurons that were spontaneously electrophysiologically active, a characteristic feature of nigrostriatal dopaminergic neurons in vivo. Differentiation was as efficient as in macroscopic culture, with up to 19% of differentiated neurons immunoreactive for tyrosine hydroxylase, the penultimate enzyme in the synthesis of dopamine. This new microfluidic cell culture model integrates the latest innovations in developmental biology and microfluidic cell culture to generate a biologically realistic and economically efficient route to personalised drug discovery for Parkinson's disease.

Graphical abstract: Differentiation of neuroepithelial stem cells into functional dopaminergic neurons in 3D microfluidic cell culture

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Publication details

The article was received on 12 Feb 2015, accepted on 30 Mar 2015 and first published on 30 Mar 2015


Article type: Paper
DOI: 10.1039/C5LC00180C
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Citation: Lab Chip, 2015,15, 2419-2428

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    Differentiation of neuroepithelial stem cells into functional dopaminergic neurons in 3D microfluidic cell culture

    E. L. Moreno, S. Hachi, K. Hemmer, S. J. Trietsch, A. S. Baumuratov, T. Hankemeier, P. Vulto, J. C. Schwamborn and R. M. T. Fleming, Lab Chip, 2015, 15, 2419
    DOI: 10.1039/C5LC00180C

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