Issue 3, 2015

Dynamic acoustic field activated cell separation (DAFACS)


Advances in diagnostics, cell and stem cell technologies drive the development of application-specific tools for cell and particle separation. Acoustic micro-particle separation offers a promising avenue for high-throughput, label-free, high recovery, cell and particle separation and isolation in regenerative medicine. Here, we demonstrate a novel approach utilizing a dynamic acoustic field that is capable of separating an arbitrary size range of cells. We first demonstrate the method for the separation of particles with different diameters between 6 and 45 μm and secondly particles of different densities in a heterogeneous medium. The dynamic acoustic field is then used to separate dorsal root ganglion cells. The shearless, label-free and low damage characteristics make this method of manipulation particularly suited for biological applications. Advantages of using a dynamic acoustic field for the separation of cells include its inherent safety and biocompatibility, the possibility to operate over large distances (centimetres), high purity (ratio of particle population, up to 100%), and high efficiency (ratio of separated particles over total number of particles to separate, up to 100%).

Graphical abstract: Dynamic acoustic field activated cell separation (DAFACS)

Supplementary files

Article information

Article type
30 Sep 2014
21 Nov 2014
First published
04 Dec 2014
This article is Open Access
Creative Commons BY license

Lab Chip, 2015,15, 802-810

Author version available

Dynamic acoustic field activated cell separation (DAFACS)

G. D. Skotis, D. R. S. Cumming, J. N. Roberts, M. O. Riehle and A. L. Bernassau, Lab Chip, 2015, 15, 802 DOI: 10.1039/C4LC01153H

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