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Issue 4, 2015
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Three-part differential of unlabeled leukocytes with a compact lens-free imaging flow cytometer

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Abstract

A compelling clinical need exists for inexpensive, portable haematology analyzers that can be utilized at the point-of-care in emergency settings or in resource-limited settings. Development of a label-free, microfluidic blood analysis platform is the first step towards such a miniaturized, cost-effective system. Here we assemble a compact lens-free in-line holographic microscope and employ it to image blood cells flowing in a microfluidic chip, using a high-speed camera and stroboscopic illumination. Numerical reconstruction of the captured holograms allows classification of unlabeled leukocytes into three main subtypes: lymphocytes, monocytes and granulocytes. A scale-space recognition analysis to evaluate cellular size and internal complexity is also developed and used to build a 3-part leukocyte differential. The lens-free image-based classification is compared to the 3-part white blood cell differential generated by using a conventional analyzer on the same blood sample and is found to be in good agreement with it.

Graphical abstract: Three-part differential of unlabeled leukocytes with a compact lens-free imaging flow cytometer

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Article information


Submitted
25 Sep 2014
Accepted
13 Dec 2014
First published
15 Dec 2014

Lab Chip, 2015,15, 1123-1132
Article type
Paper

Three-part differential of unlabeled leukocytes with a compact lens-free imaging flow cytometer

D. Vercruysse, A. Dusa, R. Stahl, G. Vanmeerbeeck, K. de Wijs, C. Liu, D. Prodanov, P. Peumans and L. Lagae, Lab Chip, 2015, 15, 1123
DOI: 10.1039/C4LC01131G

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