Jump to main content
Jump to site search

Issue 10, 2015
Previous Article Next Article

Laser ablation low-flow ICP-MS for elemental bioimaging

Author affiliations

Abstract

A laser ablation system (LA) was coupled to an in-house developed low-flow inductively coupled plasma ion source for mass spectrometry (ICP-MS). In this setup, the low-flow torch showed best analytical performance at a total argon gas flow rate of only 1.27 L min−1 and a generator power of 900 W. The optimized system was applied to elemental bioimaging. Two different sections of one kidney sample were analyzed and images of the elements Al and Br from the staining agents hematoxylin and eosin, respectively, and I from a contrast agent iodixanol were recorded selectively and with high sensitivity. For detection of the elements, the mass traces 27Al, 79Br, 81Br, and 127I were selected to demonstrate the system's analytical performance over a wide mass range. In total, the argon consumption could be reduced by >90% compared to conventional ICP-MS systems.

Graphical abstract: Laser ablation low-flow ICP-MS for elemental bioimaging

Back to tab navigation

Article information


Submitted
28 May 2015
Accepted
08 Sep 2015
First published
08 Sep 2015

J. Anal. At. Spectrom., 2015,30, 2120-2124
Article type
Paper
Author version available

Laser ablation low-flow ICP-MS for elemental bioimaging

T. Steingrobe, A. Niehoff, B. Franze, D. Lenhard, H. Pietsch, C. Engelhard, U. Karst and W. Buscher, J. Anal. At. Spectrom., 2015, 30, 2120 DOI: 10.1039/C5JA00199D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements