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Issue 10, 2015
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Single molecular force across single integrins dictates cell spreading

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Abstract

Cells' ability to sense and interpret mechanical signals from the extracellular milieu modulates the degree of cell spreading. Yet how cells detect such signals and activate downstream signaling at the molecular level remain elusive. Herein, we utilize tension gauge tether (TGT) platform to investigate the underlying molecular mechanism of cell spreading. Our data from both differentiated cells of cancerous and non-cancerous origin show that for the same stiff underlying glass substrates and for same ligand density it is the molecular forces across single integrins that ultimately determine cell spreading responses. Furthermore, by decoupling molecular stiffness and molecular tension we demonstrate that molecular stiffness has little influence on cell spreading. Our data provide strong evidence that links molecular forces at the cell-substrate interface to the degree of cell spreading.

Graphical abstract: Single molecular force across single integrins dictates cell spreading

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Supplementary files

Article information


Submitted
20 Mar 2015
Accepted
28 Jun 2015
First published
30 Jun 2015

Integr. Biol., 2015,7, 1265-1271
Article type
Paper

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