Issue 5, 2015

Lignocellulose pretreatment technologies affect the level of enzymatic cellulose oxidation by LPMO

Abstract

Sugarcane bagasse, corn stover, and wheat straw are among the most available resources for the production of cellulosic ethanol. For these biomasses we study the influence of pre-treatment methods on the chemical composition, as well as on the subsequent reactions of enzymatic hydrolysis and oxidation of cellulose. The applied pre-treatment methods are organosolv, hydrothermal, and alkaline. Hydrothermally pretreated wheat straw gave the highest cellulose conversion with 80% glucose yield and 0.8% oxidized cellulose products. Recent studies have shown that lignin is able to boost the activity of the cellulose oxidizing enzyme lytic polysaccharide monooxygenase (LPMO). The highest activity of LPMO was observed for the hydrothermally pretreated biomasses, which also contained the highest level of lignin. All hydrolyses were done at high dry matter levels using a commercial enzyme preparation containing hydrolytic and oxidative enzymes.

Graphical abstract: Lignocellulose pretreatment technologies affect the level of enzymatic cellulose oxidation by LPMO

Article information

Article type
Paper
Submitted
07 Nov 2014
Accepted
24 Feb 2015
First published
25 Feb 2015

Green Chem., 2015,17, 2896-2903

Author version available

Lignocellulose pretreatment technologies affect the level of enzymatic cellulose oxidation by LPMO

U. F. Rodríguez-Zúñiga, D. Cannella, R. D. C. Giordano, R. D. L. C. Giordano, H. Jørgensen and C. Felby, Green Chem., 2015, 17, 2896 DOI: 10.1039/C4GC02179G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements