Jump to main content
Jump to site search

Volume 185, 2015
Previous Article Next Article

Monitoring guanine photo-oxidation by enantiomerically resolved Ru(ii) dipyridophenazine complexes using inosine-substituted oligonucleotides

Author affiliations

Abstract

The intercalating [Ru(TAP)2(dppz)]2+ complex can photo-oxidise guanine in DNA, although in mixed-sequence DNA it can be difficult to understand the precise mechanism due to uncertainties in where and how the complex is bound. Replacement of guanine with the less oxidisable inosine (I) base can be used to understand the mechanism of electron transfer (ET). Here the ET has been compared for both Λ- and Δ-enantiomers of [Ru(TAP)2(dppz)]2+ in a set of sequences where guanines in the readily oxidisable GG step in {TCGGCGCCGA}2 have been replaced with I. The ET has been monitored using picosecond and nanosecond transient absorption and picosecond time-resolved IR spectroscopy. In both cases inosine replacement leads to a diminished yield, but the trends are strikingly different for Λ- and Δ-complexes.

Back to tab navigation

Associated articles

Article information


Submitted
19 May 2015
Accepted
01 Jul 2015
First published
01 Oct 2015

Faraday Discuss., 2015,185, 455-469
Article type
Paper
Author version available

Monitoring guanine photo-oxidation by enantiomerically resolved Ru(II) dipyridophenazine complexes using inosine-substituted oligonucleotides

P. M. Keane, F. E. Poynton, J. P. Hall, I. P. Clark, I. V. Sazanovich, M. Towrie, T. Gunnlaugsson, S. J. Quinn, C. J. Cardin and J. M. Kelly, Faraday Discuss., 2015, 185, 455
DOI: 10.1039/C5FD00085H

Social activity

Search articles by author

Spotlight

Advertisements