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Issue 14, 2015
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Qualifying label components for effective biosensing using advanced high-throughput SEIRA methodology

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Abstract

The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing efforts on nano-scaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. Nanoparticle induced sensitivity enhancement and its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are well suitable for these purposes. The potential of diverse fluorophore–antibody conjugates, being chemisorbed onto low-cost gold nanoparticulate SEIRA substrates, has been explored with respect to their spectral discriminability. These novel biolabels deliver molecular SEIRA fingerprints that have been successfully analyzed by both uni- and multivariate analyzing tools, to discriminate their multiplexing capabilities. We show that this robust spectral encoding via SEIRA fingerprints opens up new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics.

Graphical abstract: Qualifying label components for effective biosensing using advanced high-throughput SEIRA methodology

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Supplementary files

Article information


Submitted
18 Dec 2014
Accepted
02 Mar 2015
First published
03 Mar 2015

This article is Open Access

Phys. Chem. Chem. Phys., 2015,17, 9471-9479
Article type
Paper
Author version available

Qualifying label components for effective biosensing using advanced high-throughput SEIRA methodology

A. Hornemann, D. Eichert, S. Flemig, G. Ulm and B. Beckhoff, Phys. Chem. Chem. Phys., 2015, 17, 9471
DOI: 10.1039/C4CP05944A

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