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Issue 5, 2015
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Nonidentical intracellular drug release rates in Raman and fluorescence spectroscopic determination

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Abstract

Intracellular drug release rates were measured by monitoring mitoxantrone (MTX) on gold nanoparticle (AuNP) carriers by means of real-time label-free bimodal imaging with confocal Raman and fluorescence spectroscopy. The quenching nature of the MTX–AuNPs by nanometal surface energy transfer (NSET) was analyzed using the determined Stern–Volmer constant of KSV = 2.28 × 109 M−1. The amount of MTX released was estimated by both the decrease in the surface-enhanced resonance Raman scattering (SERRS) signal and the increase in the fluorescence intensity. Both SERRS and NSET provide quantitative relationships between the spectral intensities of MTX concentrations in solution. Inside live cells, the signal decay profiles of the drug release from AuNPs appeared to be faster at the beginning of the bond-breaking drug release for the SERRS (R−12) than the recovery time of the NSET (R−4 or R−6). In the first 45 min, a rather fast decay rate k of 0.0252 min−1 with a short half-life t1/2 of 27.5 min was observed, whereas the rate became significantly slower in a diffusion process, 0.0093 min−1 with a longer half-life of 101.4 min, after 45 min.

Graphical abstract: Nonidentical intracellular drug release rates in Raman and fluorescence spectroscopic determination

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Publication details

The article was received on 21 Sep 2014, accepted on 30 Oct 2014 and first published on 24 Nov 2014


Article type: Paper
DOI: 10.1039/C4CP04235B
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Citation: Phys. Chem. Chem. Phys., 2015,17, 3019-3023
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    Nonidentical intracellular drug release rates in Raman and fluorescence spectroscopic determination

    E. Ganbold, J. Yoon, D. Kim and S. Joo, Phys. Chem. Chem. Phys., 2015, 17, 3019
    DOI: 10.1039/C4CP04235B

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