A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Abstract

We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.