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Issue 9, 2016
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Amplified binding-induced homogeneous assay through catalytic cycling of analyte for ultrasensitive protein detection

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Abstract

By using the principle of binding-induced DNA assembly, we have developed a novel homogeneous assay that is able to convert an affinity protein binding event into a predesigned DNA assembly. The assembled DNA sequence can be ligated into an intact DNA strand and hundreds of DNA hairpins can be cleaved by a nicking endonuclease. Each cleavage releases a single-stranded DNA (ssDNA) probe that is initially caged in the DNA hairpin. This released ssDNA probe can then turn on the fluorescence signal by desorbing a fluorescently-labelled complementary DNA probe from graphene oxide through hybridization. We demonstrate that this homogeneous, isothermal, and amplifiable assay can be tailored to detect a number of proteins, including a cancer biomarker, human prostate specific antigen, at picomolar levels in both buffer and human serum samples.

Graphical abstract: Amplified binding-induced homogeneous assay through catalytic cycling of analyte for ultrasensitive protein detection

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Publication details

The article was received on 26 Oct 2015, accepted on 02 Dec 2015 and first published on 02 Dec 2015


Article type: Communication
DOI: 10.1039/C5CC08879H
Citation: Chem. Commun., 2016,52, 1816-1819
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    Amplified binding-induced homogeneous assay through catalytic cycling of analyte for ultrasensitive protein detection

    J. Chen, B. Deng, P. Wu, F. Li, X. Li, X. C. Le, H. Zhang and X. Hou, Chem. Commun., 2016, 52, 1816
    DOI: 10.1039/C5CC08879H

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