Issue 94, 2015
From the journal:

Chemical Communications

Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples†

Kevin D. Clark,a   Melissa M. Yamsek,b   Omprakash Nachama  and  Jared L. Anderson*a  

* Corresponding authors

a Department of Chemistry, Iowa State University, Ames, IA 50011, USA
E-mail: andersoj@iastate.edu
Tel: +1 515-294-8356

b Department of Chemistry and Biochemistry, University of Toledo, Toledo, OH 43606, USA

Abstract

A polymerase chain reaction (PCR) buffer was systematically designed to relieve the inhibition caused by hydrophobic magnetic ionic liquids (MILs). We describe a simple, rapid method for MIL-based plasmid DNA extraction from crude bacterial cell lysate in which DNA-enriched MIL is transferred directly to a PCR tube for analysis.

Graphical abstract: Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

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Article information

DOI
https://doi.org/10.1039/C5CC07253K
Article type
Communication
Submitted
29 Aug 2015
Accepted
27 Sep 2015
First published
29 Sep 2015

Chem. Commun., 2015,51, 16771-16773

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Magnetic ionic liquids as PCR-compatible solvents for DNA extraction from biological samples

K. D. Clark, M. M. Yamsek, O. Nacham and J. L. Anderson, Chem. Commun., 2015, 51, 16771 DOI: 10.1039/C5CC07253K

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