Substrate-constituted three-liquid-phase system: a green, highly efficient and recoverable platform for interfacial enzymatic reactions

Abstract

Highly efficient interfacial enzymatic hydrolysis of oil was achieved in a three-liquid-phase system, wherein the substrate constituted one of the phases. The enlarged interfacial area and relieved product inhibition were responsible for the high catalytic efficiency. Convenient product isolation and the high reusability of the enzyme were also demonstrated.