Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 22nd May 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 67, 2015
Previous Article Next Article

Fast and catalyst-free hydrazone ligation via ortho-halo-substituted benzaldehydes for protein C-terminal labeling at neutral pH

Author affiliations

Abstract

Rapid and catalyst-free hydrazone ligation reaction between ortho-halobenzaldehyde derivatives and peptide/protein hydrazides was observed at neutral pH and room temperature. 2-Chlorobenzaldehyde exhibited the fastest reaction and highest conversion rates among the series of ortho-halobenzaldehydes. The resulting hydrazone-containing bioconjugation products were also found to be fairly stable under experimental conditions. The new ligation strategy was successfully used for protein C-terminal labeling and should provide a practical approach for the modification of proteins.

Graphical abstract: Fast and catalyst-free hydrazone ligation via ortho-halo-substituted benzaldehydes for protein C-terminal labeling at neutral pH

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 May 2015, accepted on 09 Jul 2015 and first published on 09 Jul 2015


Article type: Communication
DOI: 10.1039/C5CC04382D
Chem. Commun., 2015,51, 13189-13192

  •   Request permissions

    Fast and catalyst-free hydrazone ligation via ortho-halo-substituted benzaldehydes for protein C-terminal labeling at neutral pH

    Y. Xu, L. Xu, Y. Xia, C. Guan, Q. Guo, Y. Fu, C. Wang and Y. Li, Chem. Commun., 2015, 51, 13189
    DOI: 10.1039/C5CC04382D

Search articles by author

Spotlight

Advertisements