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Issue 72, 2015
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Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

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Abstract

Gd3+ is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd3+-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.

Graphical abstract: Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

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Publication details

The article was received on 22 May 2015, accepted on 22 Jul 2015 and first published on 22 Jul 2015


Article type: Communication
DOI: 10.1039/C5CC04234H
Citation: Chem. Commun., 2015,51, 13850-13853
  • Open access: Creative Commons BY-NC license
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    Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd3+-DOTA

    F. Wojciechowski, A. Groß, I. T. Holder, L. Knörr, M. Drescher and J. S. Hartig, Chem. Commun., 2015, 51, 13850
    DOI: 10.1039/C5CC04234H

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