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We observed over 400-fold enhanced fluorescence from single Cy5 molecules assembled on multilayer silver nanoparticle–dielectric–metal (PDM) substrate. This substantial enhancement is due to the near-field enhanced excitation, emission, and interaction of Cy5 with plasmonic nanostructures. Experimental observation is supported by finite-element method calculations.

Graphical abstract: Several hundred-fold enhanced fluorescence from single fluorophores assembled on silver nanoparticle–dielectric–metal substrate

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