Daniel Heine, Srividhya Sundaram, Tom Bretschneider and Christian Hertweck
Chem. Commun., 2015,51, 9872-9875
DOI:
10.1039/C5CC03085D,
Communication
The versatility of the branching module of the rhizoxin polyketide synthase was tested in an in vitro enzyme assay with a polyketide mimic and branched (di)methylmalonyl-CoA extender units. Comparison of the products with synthetic reference compounds revealed that the module is able to stereoselectively introduce two branches in one step by a Michael addition–lactonisation sequence, thus expanding the scope of previously studied PKS systems.
