Photo-cross-linking using trifluorothymidine and 3-cyanovinylcarbazole induced a large shifted 19F MR signal†
Abstract
Photo-cross-linking of trifluorothymidine (TFT) using 3-cyanovinylcarbazole (CNVK) clearly shifted its 19F nuclear magnetic resonance (NMR) signal 8 ppm. This CNVK mediated ultrafast photo-cross-linking-induced shift can be utilized for miRNA detection by hybridization chain reaction (HCR) to detect 10 nM of a target in a sequence-specific manner.