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Issue 43, 2015
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Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

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Abstract

The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site (‘sandwich’) assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.

Graphical abstract: Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

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Article information


Submitted
19 Mar 2015
Accepted
24 Apr 2015
First published
27 Apr 2015

Chem. Commun., 2015,51, 9050-9053
Article type
Communication
Author version available

Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

O. Chumphukam, T. T. Le, S. Piletsky and A. E. G. Cass, Chem. Commun., 2015, 51, 9050
DOI: 10.1039/C5CC02314A

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