Issue 37, 2015
From the journal:

Chemical Communications

A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging†

Cassandra L. Fleming,a   Trent D. Ashton,a   Cameron Nowell,b   Mark Devlin,c   Anthony Natoli,c   Jeannette Schreudersc  and  Frederick M. Pfeffer*a  

* Corresponding authors

a Research Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Victoria, Australia
E-mail: fred.pfeffer@deakin.edu.au

b Monash Institute of Pharmaceutical Science, Royal Parade, Parkville, Victoria, Australia

c Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Victoria, Australia

Abstract

Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify ‘profluorogenic’ substrates for intracellular studies.

Graphical abstract: A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging

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Article information

DOI
https://doi.org/10.1039/C5CC02059J
Article type
Communication
Submitted
11 Mar 2015
Accepted
02 Apr 2015
First published
02 Apr 2015
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 7827-7830

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A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging

C. L. Fleming, T. D. Ashton, C. Nowell, M. Devlin, A. Natoli, J. Schreuders and F. M. Pfeffer, Chem. Commun., 2015, 51, 7827 DOI: 10.1039/C5CC02059J

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