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Issue 60, 2015
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Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription

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Abstract

Target miRNA was detected by producing RNA: rolling circle polymerization (RCP) and rolling circle transcription (RCT) were interlinked to provide dual amplification, producing multiplied malachite green (MG) aptamers, and a signal was generated by the SERS (surface-enhanced Raman scattering) quantification of the MG molecules that were bound to the transcripts.

Graphical abstract: Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription

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Supplementary files

Article information


Submitted
28 Feb 2015
Accepted
15 Jun 2015
First published
16 Jun 2015

Chem. Commun., 2015,51, 11976-11979
Article type
Communication
Author version available

Detecting miRNA by producing RNA: a sensitive assay that combines rolling-circle DNA polymerization and rolling circle transcription

X. Li, F. Zheng and R. Ren, Chem. Commun., 2015, 51, 11976 DOI: 10.1039/C5CC01748C

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