Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 22nd May 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 44, 2015
Previous Article Next Article

Achieving room temperature DNA amplification by dialling in destabilization

Author affiliations

Abstract

The ability to amplify nucleic acid biomarkers at room temperature has remained elusive despite the great need of diagnostics suitable for the point of care. To exponentially amplify DNA within a wide range of ambient temperatures (18–26 °C), we explore combining two destabilizing elements in our isothermal lesion-induced DNA amplification system. We demonstrate rapid DNA amplification at the bench without a heat source.

Graphical abstract: Achieving room temperature DNA amplification by dialling in destabilization

Back to tab navigation

Supplementary files

Publication details

The article was received on 20 Feb 2015, accepted on 20 Apr 2015 and first published on 20 Apr 2015


Article type: Communication
DOI: 10.1039/C5CC01548K
Chem. Commun., 2015,51, 9101-9104

  •   Request permissions

    Achieving room temperature DNA amplification by dialling in destabilization

    B. S. Alladin-Mustan, C. J. Mitran and J. M. Gibbs-Davis, Chem. Commun., 2015, 51, 9101
    DOI: 10.1039/C5CC01548K

Search articles by author

Spotlight

Advertisements