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Issue 44, 2015
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Achieving room temperature DNA amplification by dialling in destabilization

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Abstract

The ability to amplify nucleic acid biomarkers at room temperature has remained elusive despite the great need of diagnostics suitable for the point of care. To exponentially amplify DNA within a wide range of ambient temperatures (18–26 °C), we explore combining two destabilizing elements in our isothermal lesion-induced DNA amplification system. We demonstrate rapid DNA amplification at the bench without a heat source.

Graphical abstract: Achieving room temperature DNA amplification by dialling in destabilization

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Article information


Submitted
20 Feb 2015
Accepted
20 Apr 2015
First published
20 Apr 2015

Chem. Commun., 2015,51, 9101-9104
Article type
Communication

Achieving room temperature DNA amplification by dialling in destabilization

B. S. Alladin-Mustan, C. J. Mitran and J. M. Gibbs-Davis, Chem. Commun., 2015, 51, 9101
DOI: 10.1039/C5CC01548K

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