A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

Chunxia Song; Xiaohai Yang; Kemin Wang; Qing Wang; Jianbo Liu; Jin Huang; Leiliang He; Pei Liu; Zhihe Qing; Wei Liu

doi:10.1039/C4CC08991J

A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction†

Chunxia Song,^a Xiaohai Yang,*^a Kemin Wang,*^a Qing Wang,^a Jianbo Liu,^a Jin Huang,^a Leiliang He,^a Pei Liu,^a Zhihe Qing^a and Wei Liu^a

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* Corresponding authors

^a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China
E-mail: kmwang@hnu.edu.cn, yangxiaohai@hnu.edu.cn
Fax: +86 731 88821566
Tel: +86 731 88821566

Abstract

A strategy for T4 polynucleotide kinase activity detection was proposed based on a β-cyclodextrin polymer (polyβ-CD) and an exonuclease reaction. The fluorescence of pyrene enhanced by more than 10 times in the presence of polyβ-CD, and a simple detection of T4 PNK was achieved with a detection limit of 0.02 units per mL.

Chemical Communications

A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction†

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A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

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