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Issue 39, 2015
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Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

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Abstract

Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.

Graphical abstract: Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

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Article information


Submitted
07 Oct 2014
Accepted
05 Feb 2015
First published
05 Feb 2015

This article is Open Access

Chem. Commun., 2015,51, 8245-8248
Article type
Communication

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

R. Rakauskaitė, G. Urbanavičiūtė, A. Rukšėnaitė, Z. Liutkevičiūtė, R. Juškėnas, V. Masevičius and S. Klimašauskas, Chem. Commun., 2015, 51, 8245
DOI: 10.1039/C4CC07910H

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