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Issue 2, 2015
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Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

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Abstract

Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides.

Graphical abstract: Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

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Supplementary files

Article information


Submitted
01 Oct 2014
Accepted
30 Oct 2014
First published
04 Nov 2014

Chem. Commun., 2015,51, 409-412
Article type
Communication
Author version available

Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

F. J. Piscotta, J. M. Tharp, W. R. Liu and A. J. Link, Chem. Commun., 2015, 51, 409
DOI: 10.1039/C4CC07778D

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