Jump to main content
Jump to site search

Issue 2, 2015
Previous Article Next Article

A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity

Author affiliations

Abstract

A novel time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) was developed. We used a straightforward strategy to modify a known ALK substrate into a peptide biosensor that can accommodate terbium luminescence sensitization upon its phosphorylation by ALK. Since this strategy is generalizable, this high-throughput screening compatible assay serves as an example for development of other kinase assays that employ terbium luminescence as a read-out.

Graphical abstract: A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 Sep 2014, accepted on 30 Oct 2014 and first published on 04 Nov 2014


Article type: Communication
DOI: 10.1039/C4CC07453J
Author version
available:
Download author version (PDF)
Citation: Chem. Commun., 2015,51, 362-365
  •   Request permissions

    A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity

    W. Cui and L. L. Parker, Chem. Commun., 2015, 51, 362
    DOI: 10.1039/C4CC07453J

Search articles by author

Spotlight

Advertisements