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Issue 10, 2015
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Thermoresponsive hydrogel maintains the mouse embryonic stem cell “naïve” pluripotency phenotype

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Abstract

A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N′-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a “naïve” state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.

Graphical abstract: Thermoresponsive hydrogel maintains the mouse embryonic stem cell “naïve” pluripotency phenotype

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Supplementary files

Article information


Submitted
13 Apr 2015
Accepted
11 Jul 2015
First published
22 Jul 2015

Biomater. Sci., 2015,3, 1371-1375
Article type
Communication
Author version available

Thermoresponsive hydrogel maintains the mouse embryonic stem cell “naïve” pluripotency phenotype

C. Mangani, A. Lilienkampf, M. Roy, P. A. de Sousa and M. Bradley, Biomater. Sci., 2015, 3, 1371
DOI: 10.1039/C5BM00121H

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