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Issue 14, 2015
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A versatile probe for chemoselective capture and analysis of carbonyl compounds in exhaled breath

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Abstract

We describe an aminooxy reagent for the capture of trace aldehyde and ketone volatile organic compounds (VOCs) in exhaled breath. The reagent, 4-(2-aminooxyethyl)-morpholin-4-ium chloride (AMAH), when coated onto micropillars within a silicon microreactor, chemoselectively and covalently retains carbonyl VOCs from exhaled breath. The AMAH–carbonyl adducts are then eluted from the microreactor with methanol and directly analyzed by Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometry (MS), where the aminium ion of the reagent enhances the sensitivity for high mass accuracy. We also outline a protocol for treatment of the AMAH–carbonyl adducts with poly(4-vinylpyridine) to afford the corresponding volatile carbonyl adducts that now can be analyzed by gas chromatography-mass spectrometry (GC-MS). This convenient protocol imparts flexibility for the identification and quantification of isomeric VOCs using both FT-ICR-MS and GC-MS. Representative breath analyses are given to illustrate this applicability of AMAH.

Graphical abstract: A versatile probe for chemoselective capture and analysis of carbonyl compounds in exhaled breath

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Article information


Submitted
18 Jun 2015
Accepted
24 Jun 2015
First published
24 Jun 2015

Anal. Methods, 2015,7, 6027-6033
Article type
Paper
Author version available

A versatile probe for chemoselective capture and analysis of carbonyl compounds in exhaled breath

R. J. Knipp, M. Li, X. Fu and M. H. Nantz, Anal. Methods, 2015, 7, 6027
DOI: 10.1039/C5AY01576F

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