A simple one-step extraction method for the determination of organophosphorus pesticides in shuanghuanglian and antivirus oral liquids by gas chromatography-tandem mass spectrometry

Abstract

A new multi-residue method was proposed for the determination of 11 organophosphorus pesticides (OPPs) in Traditional Chinese medicinal preparation oral liquids. The method was based on a simple one-step extraction procedure without further clean-up step. The analytes were extracted by a solvent mixture of acetone and n-hexane, and the experimental parameters such as the ratio of acetone : n-hexane (v/v), the volume of extraction solvent and the extraction time were optimized via orthogonal array experimental design (OAD). All of the pesticides were successfully identified and quantified by gas chromatography-tandem mass spectrometry (GC-MS/MS). The linearity of calibration curves is excellent for matrix-matched standards and yields the coefficients of determination (R²) > 0.9997 for the target analytes. Low limits of quantification (10 μg L⁻¹) were achieved. Under optimized conditions, the average recoveries for most pesticides (spiked at 10, 20, and 50 μg L⁻¹) range from 70% to 120%, and RSDs are less than 13.3%. This satisfactory method has been established for the first time for the determination of OPPs in Traditional Chinese medicinal preparation oral liquids.