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Issue 16, 2015
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Fast protein detection in raw blood by size-exclusion SPR sensing

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Abstract

We have developed a simple technique for detecting protein in raw blood samples using size-exclusion surface plasmon resonance (SPR) sensing. We designed a sensor chip consisting of a microslit array fabricated on a flat gold surface with gaps that were smaller in size than a mammalian red blood cell, which was designed for use in various Kretschmann-type SPR instruments. Blood cells were blocked by the microwalls, whereas the serum reached the SPR sensing field, enabling cell filtration and immediate protein detection in a one-step operation that required only the dispensing of a blood sample onto the chip. We evaluated the influence of the chip structure on SPR characteristics, and demonstrated using a whole blood sample from horse that IgG can be detected using the chip within 100 s of application.

Graphical abstract: Fast protein detection in raw blood by size-exclusion SPR sensing

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Publication details

The article was received on 22 Apr 2015, accepted on 12 Jun 2015 and first published on 15 Jun 2015


Article type: Paper
DOI: 10.1039/C5AY01045D
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Anal. Methods, 2015,7, 6483-6488

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    Fast protein detection in raw blood by size-exclusion SPR sensing

    K. Terao, S. Hiramatsu, T. Suzuki, H. Takao, F. Shimokawa and F. Oohira, Anal. Methods, 2015, 7, 6483
    DOI: 10.1039/C5AY01045D

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