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Issue 17, 2015
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Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

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Abstract

Cell collection based on deterministic lateral displacement (DLD) and cell circulation with a loop channel are two component technologies for stressless cell retention which have been developed with a view to working toward suspension culture in a microfluidic channel. DLD devices with low array angles collect floating S. cerevisiae through an array effectively. The DLD device with an array angle of 2.6° showed an efficiency of 91.7%. Two types of loop channels with a piezoelectrie pump make a stable two-phase laminar flow of a pre-filled fluid and supplied fluid. A loop channel with a connection between an inlet and outlet on the opposite side replaces a filled fluid in the channel with a supplied fluid, which is essential for supplying nutrient rich medium to cells in microfluidic channels as well as retaining cells in a microenvironment without external stresses.

Graphical abstract: Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

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Publication details

The article was received on 24 Feb 2015, accepted on 14 Apr 2015 and first published on 06 May 2015


Article type: Paper
DOI: 10.1039/C5AY00485C
Anal. Methods, 2015,7, 7264-7269

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    Hydrodynamic nonadhesive cell retention in a microfluidic circuit for stressless suspension culture

    T. Naito, N. Kaji, M. Tokeshi, T. Kubo, Y. Baba and K. Otsuka, Anal. Methods, 2015, 7, 7264
    DOI: 10.1039/C5AY00485C

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