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Issue 7, 2015
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Evaluation of different functional groups for covalent immobilization of enzymes in the development of biosensors with oxygen optical transduction

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Abstract

Four different types of polymeric particles with different functional groups on their surface have been evaluated to develop biosensors using glucose oxidase as a model enzyme. Direct covalent immobilization was achieved on particles functionalized with chloride, epoxy and vinyl groups via the reactive functional groups on the surface, whereas particles functionalized with carboxylic groups, used as reference materials, were pre-activated with carbodiimide. Immobilization was successfully performed under very mild conditions (20 °C, pH 8.0). In order to determine the advantages and disadvantages of each functional group, both the amounts of immobilized enzymes and their relative activities were fully investigated. In order to demonstrate their applicability on the design on biosensing with oxygen optical transduction, the functionalized particles were co-immobilized in gold chips with oxygen sensing particles (PSMA–PtTFPP) using electrophoretic deposition and characterised for glucose determination in solution.

Graphical abstract: Evaluation of different functional groups for covalent immobilization of enzymes in the development of biosensors with oxygen optical transduction

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Publication details

The article was received on 13 Jan 2015, accepted on 08 Feb 2015 and first published on 09 Feb 2015


Article type: Paper
DOI: 10.1039/C5AY00103J
Anal. Methods, 2015,7, 2943-2949
  • Open access: Creative Commons BY-NC license
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    Evaluation of different functional groups for covalent immobilization of enzymes in the development of biosensors with oxygen optical transduction

    T. Ramon-Marquez, A. L. Medina-Castillo, J. F. Fernandez-Sanchez and A. Fernández-Gutiérrez, Anal. Methods, 2015, 7, 2943
    DOI: 10.1039/C5AY00103J

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