Issue 6, 2015

Modulated fluorescence detection with microelectromagnetic traps

Abstract

Microelectromagnetic traps (METs) were utilized to concentrate streptavidin modified magnetic particles from a microfluidic analyte stream. Particle capture was detected by bioconjugation either to biotinylated quantum dots or to DNA hybrids labeled with biotin and fluorescein. Subsequent modulation of the MET trapping field resulted in modulated fluorescence intensity at the MET. Detection of pM concentrations of streptavidin bound to magnetic particles and fM concentrations of DNA was possible through Fourier analysis of the modulated fluorescence response. This detection strategy represents a suitable approach to signal enhancement for magnetic pull-down assays and related technologies.

Graphical abstract: Modulated fluorescence detection with microelectromagnetic traps

Article information

Article type
Communication
Submitted
27 Nov 2014
Accepted
11 Feb 2015
First published
27 Feb 2015

Anal. Methods, 2015,7, 2273-2277

Author version available

Modulated fluorescence detection with microelectromagnetic traps

R. Zakeri, J. R. Basore and L. A. Baker, Anal. Methods, 2015, 7, 2273 DOI: 10.1039/C4AY02828G

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