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Issue 17, 2015
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A non-destructive test to assess the axial heterogeneity of in situ modified monoliths for HPLC

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Abstract

This paper describes a non-destructive “peak parking” protocol in order to assess the axial heterogeneity of an in situ modified monolithic column for high performance liquid chromatography; a “gradient stationary phase” was designed whereby the ligand density decreases along the length of the rod in the “forward flow” configuration. The results of multi-location peak parking demonstrated a consistent increase in peak variance from the 1 cm position of the column to the 9 cm location. This increase in band broadening supported the theory of a decreasing ligand density along the length of this gradient column. This is consistent with efficiency measurements performed in both the forward and reverse flow directions, with an improved efficiency (15% increase in N m−1) in the reverse direction. These results are consistent with theoretical investigations into stationary phase gradients.

Graphical abstract: A non-destructive test to assess the axial heterogeneity of in situ modified monoliths for HPLC

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Article information


Submitted
25 Nov 2014
Accepted
30 Jan 2015
First published
30 Jan 2015

Anal. Methods, 2015,7, 7177-7185
Article type
Paper
Author version available

A non-destructive test to assess the axial heterogeneity of in situ modified monoliths for HPLC

D. N. Bassanese, A. Soliven, X. A. Conlan, R. A. Shalliker, N. W. Barnett and P. G. Stevenson, Anal. Methods, 2015, 7, 7177
DOI: 10.1039/C4AY02812K

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