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Issue 5, 2015
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Thermoelectric lab-on-a-chip ELISA

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Abstract

We report a new, thermoelectric method for performing enzyme-linked immunosorbent assay (ELISA) in a microfluidic device. The concentration of the analyte is determined by measuring the heat of an enzymatic reaction between glucose and glucose oxidase using thin-film antimony/bismuth thermopile. The feasibility of lab-on-a-chip thermoelectric ELISA is demonstrated by measuring the concentration of 8-hydroxy-2-deoxyguanosine (8OHdG) in urine samples from amyloid precursor protein transgenic mice. The detection method is based on formation of a complex between 8OHdG, anti-8OHdG capture antibody and glucose oxidase linked IgG antibody. The complex is immobilized at the lower channel wall of the microfluidic device, over the measuring junctions of the thermopile. The amount of heat detected by the thermoelectric sensor is inversely proportional to the concentration of 8OHdG. Standard calibration curve was created using synthetic 8OHdG. The regression line equation of the standard calibration curve was used to estimate the concentration of 8OHdG in mouse urine.

Graphical abstract: Thermoelectric lab-on-a-chip ELISA

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Publication details

The article was received on 19 Nov 2014, accepted on 12 Jan 2015 and first published on 29 Jan 2015


Article type: Paper
DOI: 10.1039/C4AY02764G
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Anal. Methods, 2015,7, 2055-2063

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    Thermoelectric lab-on-a-chip ELISA

    G. G. Nestorova, V. L. Kopparthy, N. D. Crews and E. J. Guilbeau, Anal. Methods, 2015, 7, 2055
    DOI: 10.1039/C4AY02764G

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