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Issue 3, 2015
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Integrated hybrid polystyrene–polydimethylsiloxane device for monitoring cellular release with microchip electrophoresis and electrochemical detection

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Abstract

In this work, a polystyrene (PS)–polydimethylsiloxane (PDMS) hybrid device was developed to enable the integration of cell culture with analysis by microchip electrophoresis and electrochemical detection. It is shown that this approach combines the fundamental advantages of PDMS devices (the ability to integrate pumps and valves) and PS devices (the ability to permanently embed fluidic tubing and electrodes). The embedded fused-silica capillary enables high temporal resolution measurements from off-chip cell culture dishes and the embedded electrodes provide close to real-time analysis of small molecule neurotransmitters. A novel surface treatment for improved (reversible) adhesion between PS and PDMS is described using a chlorotrimethylsilane stamping method. It is demonstrated that a Pd decoupler is efficient at handling the high current (and cathodic hydrogen production) resulting from use of high ionic strength buffers needed for cellular analysis; thus allowing an electrophoretic separation and in-channel detection. The separation of norepinephrine (NE) and dopamine (DA) in highly conductive biological buffers was optimized using a mixed surfactant system. This PS–PDMS hybrid device integrates multiple processes including continuous sampling from a cell culture dish, on-chip pump and valving technologies, microchip electrophoresis, and electrochemical detection to monitor neurotransmitter release from PC 12 cells.

Graphical abstract: Integrated hybrid polystyrene–polydimethylsiloxane device for monitoring cellular release with microchip electrophoresis and electrochemical detection

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Publication details

The article was received on 28 Oct 2014, accepted on 20 Dec 2014 and first published on 14 Jan 2015


Article type: Paper
DOI: 10.1039/C4AY02569E
Anal. Methods, 2015,7, 884-893

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    Integrated hybrid polystyrene–polydimethylsiloxane device for monitoring cellular release with microchip electrophoresis and electrochemical detection

    A. S. Johnson, B. T. Mehl and R. S. Martin, Anal. Methods, 2015, 7, 884
    DOI: 10.1039/C4AY02569E

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