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Issue 20, 2015
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Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections

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Abstract

Ion mobility spectrometry techniques (IMS and IMS-IMS) combined with collision-induced dissociation (CID) and mass spectrometry (MS) are used to investigate the structures of singly-lithiated carbohydrate isomers. With the exception of some favorable cases, IMS-MS analyses of underivatized carbohydrates reveal that most isobaric precursor ions have similar collision cross sections (ccs). In contrast, ccs values for isomeric fragment ions obtained by IMS-CID-IMS-MS analysis are often different, and thus appear to be useful as a means of distinguishing the isomeric precursors. We report values of ccs (in He) for precursor- and associated-fragment ions for three monosaccharide isomers (glucose, galactose and fructose), ten disaccharide isomers (sucrose, leucrose, palatinose, trehalose, cellobiose, β-gentiobiose, isomaltose, maltose, lactose and melibiose), and three trisaccharide isomers (raffinose, melezitose and maltotriose). These values are discussed as a means of differentiating precursor carbohydrates.

Graphical abstract: Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections

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Publication details

The article was received on 28 Apr 2015, accepted on 07 Aug 2015 and first published on 07 Aug 2015


Article type: Paper
DOI: 10.1039/C5AN00840A
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Analyst, 2015,140, 6922-6932

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    Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections

    M. M. Gaye, R. Kurulugama and D. E. Clemmer, Analyst, 2015, 140, 6922
    DOI: 10.1039/C5AN00840A

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