Jump to main content
Jump to site search

Issue 12, 2015
Previous Article Next Article

Cold shock induces apoptosis of dorsal root ganglion neurons plated on infrared windows

Author affiliations

Abstract

The chemical status of live sensory neurons is accessible with infrared microspectroscopy of appropriately prepared cells. In this paper, individual dorsal root ganglion (DRG) neurons have been prepared with two different protocols, and plated on glass cover slips, BaF2 and CaF2 substrates. The first protocol exposes the intact DRGs to 4 °C for between 20–30 minutes before dissociating individual neurons and plating 2 hours later. The second protocol maintains the neurons at 23 °C for the entire duration of the sample preparation. The visual appearance of the neurons is similar. The viability was assessed by means of trypan blue exclusion method to determine the viability of the neurons. The neurons prepared under the first protocol (cold exposure) and plated on BaF2 reveal a distinct chemical signature and chemical distribution that is different from the other sample preparations described in the paper. Importantly, results for other sample preparation methods, using various substrates and temperature protocols, when compared across the overlapping spectral bandwidth, present normal chemical distribution within the neurons. The unusual chemically specific spatial variation is dominated by a lack of protein and carbohydrates in the center of the neurons and signatures of unraveling DNA are detected. We suggest that cold shock leads to apoptosis of DRGs, followed by osmotic stress originating from ion gradients across the cell membrane leading to cell lysis.

Graphical abstract: Cold shock induces apoptosis of dorsal root ganglion neurons plated on infrared windows

Back to tab navigation

Publication details

The article was received on 15 Apr 2015, accepted on 13 May 2015 and first published on 15 May 2015


Article type: Paper
DOI: 10.1039/C5AN00729A
Author version
available:
Download author version (PDF)
Citation: Analyst, 2015,140, 4046-4056
  • Open access: Creative Commons BY license
  •   Request permissions

    Cold shock induces apoptosis of dorsal root ganglion neurons plated on infrared windows

    E. Aboualizadeh, E. C. Mattson, C. L. O'Hara, A. K. Smith, C. L. Stucky and C. J. Hirschmugl, Analyst, 2015, 140, 4046
    DOI: 10.1039/C5AN00729A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements