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Issue 13, 2015
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DNA sensor's selectivity enhancement and protection from contaminating nucleases due to a hydrated ionic liquid

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Abstract

The thermodynamic stability of certain mismatched base pairs has made the development of DNA sequence sensing systems challenging. Thus, the stability of fully matched and mismatched DNA oligonucleotides in the hydrated ionic liquid choline dihydrogen phosphate (choline dhp) was investigated. Mismatched base pairs were significantly destabilized in choline dhp relative to those in aqueous buffer. A molecular beacon that forms a triplex with a conserved HIV-1 sequence was then designed and tested in choline dhp. The molecular beacon specifically detected the target duplex via triplex formation at concentrations as low as 1 pmol per 10 μL with 10 000-fold sequence selectivity. Moreover, the molecular beacon was protected from a contaminating nuclease in choline dhp, and DNAs in aqueous solutions were not sufficiently stable for practical use.

Graphical abstract: DNA sensor's selectivity enhancement and protection from contaminating nucleases due to a hydrated ionic liquid

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Supplementary files

Article information


Submitted
19 Mar 2015
Accepted
15 Apr 2015
First published
15 Apr 2015

Analyst, 2015,140, 4393-4398
Article type
Paper
Author version available

DNA sensor's selectivity enhancement and protection from contaminating nucleases due to a hydrated ionic liquid

H. Tateishi-Karimata, S. Pramanik and N. Sugimoto, Analyst, 2015, 140, 4393
DOI: 10.1039/C5AN00545K

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