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Issue 10, 2015
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A simple assay for glutathione in whole blood

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Abstract

A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.

Graphical abstract: A simple assay for glutathione in whole blood

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Article information


Submitted
20 Feb 2015
Accepted
16 Apr 2015
First published
17 Apr 2015

Analyst, 2015,140, 3339-3342
Article type
Communication
Author version available

A simple assay for glutathione in whole blood

L. Hakuna, B. Doughan, J. O. Escobedo and R. M. Strongin, Analyst, 2015, 140, 3339
DOI: 10.1039/C5AN00345H

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