pFe3+ determination of multidentate ligands by a fluorescence assay

Abstract

The fluorescence intensity of the iron–CP691 complex in the presence of a competing multidentate ligand is associated with pFe³⁺ of the competing ligand and the relative fluorescence has a linear correlation with the pFe³⁺ values. A correlation was also found to exist between the relative fluorescence and the ratio of a competing ligand to the probe CP691. Based on this assay, the pFe³⁺ value of a range of hexadentate ligands, dendrimers and polymers can be determined when they fall in the range 24.5–30.5. Only small quantities of chelators are required for this assay.