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Issue 14, 2015
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Molecular weight characterization of single globular proteins using optical nanotweezers

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Abstract

We trap a set of molecular weight standard globular proteins using a double nanohole optical trap. The root mean squared variation of the trapping laser transmission intensity gives a linear dependence with the molecular weight, showing the potential for analysis of globular proteins. The characteristic time of the autocorrelation of the trapping laser intensity variations scales with a −2/3 power dependence with the volume of the particle. A hydrodynamic laser tweezer model is used to explain these dependencies. Since this is a single particle technique that operates in solution and can be used to isolate an individual particle, we believe that it provides an interesting alternative to existing analysis methods and shows promise to expand the capabilities of protein related studies to the single particle level.

Graphical abstract: Molecular weight characterization of single globular proteins using optical nanotweezers

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Publication details

The article was received on 07 Jan 2015, accepted on 20 Feb 2015 and first published on 20 Feb 2015


Article type: Communication
DOI: 10.1039/C5AN00026B
Analyst, 2015,140, 4799-4803

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    Molecular weight characterization of single globular proteins using optical nanotweezers

    S. Wheaton and R. Gordon, Analyst, 2015, 140, 4799
    DOI: 10.1039/C5AN00026B

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