Jump to main content
Jump to site search

Issue 7, 2015
Previous Article Next Article

SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α

Author affiliations

Abstract

The intracellular pH plays an important role in various cellular processes. In this work, we describe a method for monitoring of the intracellular pH in endothelial cells by using surface enhanced Raman spectroscopy (SERS) and 4-mercaptobenzoic acid (MBA) anchored to gold nanoparticles as pH-sensitive probes. Using the Raman microimaging technique, we analysed changes in intracellular pH induced by buffers with acid or alkaline pH, as well as in endothelial inflammation induced by tumour necrosis factor-α (TNFα). The targeted nanosensor enabled spatial pH measurements revealing distinct changes of the intracellular pH in endosomal compartments of the endothelium. Altogether, SERS-based analysis of intracellular pH proves to be a promising technique for a better understanding of intracellular pH regulation in various subcellular compartments.

Graphical abstract: SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Oct 2014, accepted on 20 Nov 2014 and first published on 08 Dec 2014


Article type: Paper
DOI: 10.1039/C4AN01988A
Citation: Analyst, 2015,140, 2321-2329

  •   Request permissions

    SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α

    A. Jaworska, L. E. Jamieson, K. Malek, C. J. Campbell, J. Choo, S. Chlopicki and M. Baranska, Analyst, 2015, 140, 2321
    DOI: 10.1039/C4AN01988A

Search articles by author

Spotlight

Advertisements