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Confocal Raman microspectroscopy was used in this study to identify hepatic stellate cells (HSCs) from healthy mice and mice with untreated and treated liver steatosis. We have identified the main form of occurrence of vitamin A in healthy liver and confirmed its absence in the pathological state. Additionally, we have reported the reappearance of vitamin A in the tissue after treatment of liver steatosis.

Graphical abstract: Raman spectroscopic studies of vitamin A content in the liver: a biomarker of healthy liver

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