Jump to main content
Jump to site search

Issue 11, 2015
Previous Article Next Article

Influence of the hydrophilic–hydrophobic contrast of porous surfaces on the enzymatic performance

Author affiliations

Abstract

Herein we report the dependency of the performance (uptake, activity, stability) of hydrophilic glucose-6-phosphate dehydrogenase (G6PDH) onto mesoporous cellular foams (MCF) grafted with aminosilanes of different chain length. The resulting hydrophobic–hydrophilic contrast was carefully evaluated by combined argon and water sorption and quantified with a newly developed hydrophilicity index suitable for the characterization of large-pore materials. The enzymatic behaviour was influenced by electrostatic and hydrophobic interactions between both reaction partners due to the creation of specific microenvironments inside the pore system. The microenvironment created by the materials with high hydrophobicity did not contribute to the beneficial electrostatic interactions of the charged amine groups by increased hydrophobic forces instead a competition of both forces was present. Thus an improved biocatalytic performance was not observed for materials with high hydrophobicity but short-chain functionalized MCFs led to highly stable and active biocatalysts.

Graphical abstract: Influence of the hydrophilic–hydrophobic contrast of porous surfaces on the enzymatic performance

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 Oct 2014, accepted on 02 Feb 2015 and first published on 03 Feb 2015


Article type: Paper
DOI: 10.1039/C4TB01700E
J. Mater. Chem. B, 2015,3, 2341-2349

  •   Request permissions

    Influence of the hydrophilic–hydrophobic contrast of porous surfaces on the enzymatic performance

    D. I. Fried, D. Bednarski, M. Dreifke, F. J. Brieler, M. Thommes and M. Fröba, J. Mater. Chem. B, 2015, 3, 2341
    DOI: 10.1039/C4TB01700E

Search articles by author

Spotlight

Advertisements