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Issue 27, 2015
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Reply to the ‘Comment on “Cholesterol Solubility Limit in Lipid Membranes probed by Small Angle Neutron Scattering and MD simulations”’ by R. Epand, Soft Matter, 2015, 11, DOI: 10.1039/C4SM02819H

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Abstract

In the comment by Epand et al. on our recent article, it is stated that the term “cholesterol solubility limit” is misused. As Epand et al. point out, there is extensive literature on cholesterol phase separation in phospholipid bilayers and this term is used to define the appearance of cholesterol crystals. Moreover, as they state, this does not preclude them from existing as bilayered crystals or cholesterol-only domains within the membrane itself. Since our SANS data directly measured the maximum amount of cholesterol harboured by POPC and POPS membranes, it may have been more appropriate to use the term “cholesterol saturation limit”. Nonetheless, we stated that the saturation and solubility limits of cholesterol coincide in both POPC and POPS. Epand and et al. suggest that the data shown was insufficient to uphold this claim. Herein, we present data that supports the coincidence of cholesterol’s saturation limit with cholesterol’s solubility limit in 100 nm POPS unilamellar vesicles, where previously it has been reported to not be the case.

Graphical abstract: Reply to the ‘Comment on “Cholesterol Solubility Limit in Lipid Membranes probed by Small Angle Neutron Scattering and MD simulations”’ by R. Epand, Soft Matter, 2015, 11, DOI: 10.1039/C4SM02819H

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Article information


Submitted
05 May 2015
Accepted
05 Jun 2015
First published
17 Jun 2015

Soft Matter, 2015,11, 5582-5584
Article type
Comment
Author version available

Reply to the ‘Comment on “Cholesterol Solubility Limit in Lipid Membranes probed by Small Angle Neutron Scattering and MD simulations”’ by R. Epand, Soft Matter, 2015, 11, DOI: 10.1039/C4SM02819H

N. Krzyzanowski, L. Porcar, S. Garg, P. Butler, F. Castro-Roman, P. Jesus Bautista and U. Perez-Salas, Soft Matter, 2015, 11, 5582
DOI: 10.1039/C5SM01071C

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